Metabolic profiling of a Corynebacterium glutamicum prpD2 mutant strain
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
HUPO 2011 #PP729 |
OTOF, GC/MS |
| Year | Products |
2011 |
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| Author | |
A. Barsch1, M. Persicke2, J. Plassmeier2, Stuart Pengelley1, G. Zurek1, K. Niehaus2 1Bruker Daltonik GmbH, Bremen, Germany 2Centrum für Biotechnologie, Universität Bielefeld, Germany |
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| Reference | |
HUPO 2011 #PP729 |
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Abstract |
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• PCA analysis of GC-APCI-TOF-MS data revealed several compounds elevated in C. glutamicumextracts upon addition of propionate.• 2-Methylcitric acid and alanine were identified using accurate mass and isotopic pattern information in MS and MS/MS spectra -proof of concept for the identification of target compounds using high resolution MS technology. • TOF instrument performance (mass accuracy, resolution and isotopic fidelity) are independent of the acquisition rate. Therefore these systems are perfectly suited for coupling to gas chromatography. |
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