Isotopically Resolved FT-ICR Mass Spectrometry of Proteins Above 100 kDa at 7 Tesla
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
HUPO 2011 #P1588 |
FTMS |
| Year | Products |
2011 |
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| Author | |
Eugene N. Nikolaey1,2,3, Ivan A. Boldin1, Roland Jertz4, Jens Fuchser4, Claudia Kriete4, Gökhan Baykut4 1) The Institute for Energy Problems of Chemical PhysicsRussian Academy of Sciences, Leninskij pr. 38, k.2 MoscowRussia 119334 2) Institute of Biochemical Physics Russian Academy of Sciences, Kosygina 4, Moscow Russia 3) The Institute of Biomedical Chemistry Russian Academy ofMedical Sciences, Pogodiskaja 10 Moscow Russia 119121 4) Bruker Daltonik GmbH, Fahrenheitstrasse 4, 28359Bremen, Germany |
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| Reference | |
HUPO 2011 #P1588 |
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Abstract |
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In the dynamically harmonized ICR cell the axial quadratic distribution of the electric potential is achieved by shaping the excitation and de-tection electrodes. Resolving powers achieved at 147 kDa clearly demonstrate that the cell has a potential to analyze much higher masses and perform top-down pro-tein analysis. Ultra high resolution was also routinely achieved for small peptides [2] such as substance P in a moderate magnetic field of 7 Tesla. Mass isolated 13C peak clusters of substance P are shown in Figure 4. In the fourth 13C Cluster group 14 isotopes could be determined (not all are labeled). Resolved isotopic cluster allow a direct calculation of the elemental composition of the sample. |
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