Identification of 2000 proteins in single LCMSMS Runs from a cell line lysate
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
HUPO 2011 #A101 |
MALDI-TOF, Ion Trap, OTOF |
| Year | Products |
2011 |
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| Author | |
Andrea Schneider, Andrea Kiehne, Markus Meyer, Arnd Ingendoh, Arndt Asperger, Markus Lubeck, Stepahnie Kasper, Carsten Baessmann Bruker Daltonik, Bremen, Germany |
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| Reference | |
HUPO 2011 #A101 |
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Abstract |
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A distinct goal in proteomics is still the survey of an entire cell lysate with a maximum number of unambiguously identified proteins. While high resolution instruments are favored for this application, recent improvements in both MS instrumentation as well as in nanoLCseparation lead to a much higher number of identified proteins from such a sample in single LC/MS/MS experiments in general. Important criteria for a successful analysis are a high dynamic range, fast MS/MS and good mass accuracy. Results for various mass spectrometer platforms are demonstrated. |
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